Keragaman Gen Myxovirus (Mx) pada Itik di Kabupaten Indragiri Hulu Provinsi  Riau

Restu Misrianti; Elfawati Elfawati; Apri Irianto

Restu Misrianti Program Studi Peternakan, Fakultas Pertanian dan Peternakan, Universitas Islam Negeri Sultan Syarif Kasim Riau,
Elfawati Elfawati Program Studi Peternakan, Fakultas Pertanian dan Peternakan, Universitas Islam Negeri Sultan Syarif Kasim Riau
Apri Irianto Jurusan Peternakan Fakultas Pertanian Universitas Musamus

Keywords: Mx Gene, Duck, PCR-RFLP

Abstract

The Mx gene (Myxovirus) is a gene that functions to control the immunity of poultry against viral
diseases. This gene is a native antiviral and is able to respond to influenza virus infections such
as avian influenza. This study aims to identify the diversity of the Mx gene in ducks using the
PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) molecular
technique. The number of samples used were thirteen ducks from Indragiri Hulu Regency (Riau
Province). The restriction site of the Mx gene using the Rsa1 enzyme is in the base sequence
GTN|NAC. There are two alleles namely A and G, and three genotypes, AA, AG and GG. The
results showed that the Mx gene in ducks was monomorphic. The frequency of the resistant allele
(A) is 0%, while the frequency of the susceptible allele (G) is 100%.

References

Andreas, E., C. Sumantri, H. Nuraini, A. Farajallah and A. Anggraeni (2010). Identification of
GH|AluI and GHR|AluI Genes Polymorphisms in Indonesian Buffalo. J. of Indon. Trop.
Anim. Agric. 35:215-221.
Elfidasari,D, D D Solihin, R D Soejoedono and S Murtini (2013). Identification of Gene
Resistance to Avian Influenza Virus (Mx Gene) among Wild Waterbirds. Makara Journal
of Science. 17/1:6-10
Haller O, Frese M and Kochs G (1998). Mx proteins: mediators of innate resistance to RNA
viruses. Revue Scientific et Technique 17: 220â€“230.
Ko JH, Jin HK, Asano A, Takada A. (2002). Polymorphisms and the differential antiviral activity
of the chicken Mx gene. Genome Res. 12: 595-601.
Mamutse J, Gunawan A, Sumantri C, Murtini S, Sartika T. (2018). Association of the toll-like
receptor 4 (TLR4) and myxovirus (Mx) genes with resistance toSalmonella and
Newcastle Disease in selected sentul chickens. Int J Poul Sci.17(12): 591â€“599.
Nei, M, Kumar S. 2000. Molecular Evolutionary Genetics. New York: Columbia University Press
Qu LJ, Lia XY, Xu GY, Ning ZH, Yang N. (2009). Lower antibody response in chickens
homozygous for the Mx resistant allele to avian influenza. AsianAust J Anim Sci. 22(4):
465â€“470
Seyama T, Ko JH, Ohe M, and Sasaoka N.(2006). Population research of genetic polymorphism
at amino acid position 631 in chicken Mx protein with differential antiviral activity.
Biochem. Genet. 44: 437-448.